Outline the mechanism of action of ampicillin, gentamicin, vancomycin and ciprofloxacin.
How does resistance develop to each of these antibiotics?
The question could have been answered succinctly using a table. Better answers included detail
of how antibiotics inhibited enzymes, effects on ribosomal subunits, and impact on cell wall
synthesis or inhibit DNA gyrase. The mechanism of resistance required an understanding of
beta-lactam inhibition, gene mutation altering target enzymes and ribosomal subunits, and efflux
mechanisms. Mention of Van A and Van B phenotypes was also sought.