Beta-blockers

This chapter deals with beta-blockers, and all the drugs with mixed antisympathetic effects which we tend to group with β-receptor antagonists. It is probably relevant to Section G8(iii)  of the 2017 CICM Primary Syllabus, which asks the exam candidate to "understand the pharmacology of anti-hypertensive drugs". This topic has appeared several times in the past papers, with the questions taking on a predictable compare-em-up character. Specifically, metoprolol esmolol and carvedilol have all been singled out as important to critical care. In addition to these, the chapter covers a couple of others (atenolol, bisoprolol, nebivolol, labetalol) because they get a fair amount of use and are therefore worth knowing something about. Other drugs properly belonging to this class were omitted in some sort of arbitrary and unscientific process emblematic of the author's undisciplined mind, where they were viewed as either too boring (timolol, eye drops, erh) or better suited to the antiarrhythmic section (sotalol).

Anyway, the past exam content which is relevant here consists of the following SAQs:

  • Question 5 from the first paper of 2022 (labetalol vs. esmolol)
  • Question 14 from the second paper of 2019 (compare metoprolol and esmolol)
  • Question 10 from the first paper of 2013 (carvedilol and spironolactone, weirdly)
  • Question 4(p.2) from the first paper of 2010 (metoprolol and GTN)

These questions lend themselves to a tabulated format, but unfortunately there are too many drugs on the list to make a neat table. So, instead, the summary of the class is offered here. 

Beta-Blockers: Class Rules and their Exceptions

Domain Rule Exceptions

Administration
& absorption

All beta-blockersare enterally administered
  • Esmolol
All beta blockers have great GI absopriton
  • Esmolol 
  • Atenolol
Distribution Most of these drugs have a relatively large volume of distribution, and are highly protein-bound
  • Atenolol
Solubility Most of these drugs are hihgly lipid-soluble
  • Esmolol
  • Atenolol
Metabolism All beta blockers ungergo extensive hepatic metabolism, except...
  • Atenolol
  • Esmolol
Clearance None are dependent on renal excretion, except
  • Atenolol
  • Sotalol
Mechanism of action
  • By binding to Gs-protein coupled β1 receptors, block cAMP synthesis and thus decrease intracellular calcium concentration, thereby decreasign contractility
  • Decrease automatic self-depolarisation by affecting cAMP-sensitive If current in pacemaker cells 

Some also have:

  • α1 effects: afterload reduction, vasodilation (eg. carvedilol, labetalol)
  • Membrane-stabilising (sodium channel blocker) effect, eg. propanolol
  • Instrinsic sympathomimetic activity (eg. acetbutalol)
Clinical effects
  • β1 effects: decreased heart rate, decreased contractility, decreased blood pressure, lower myocardial oxygen demand and increased diastolci coronary fillng, and decreased arrhythmogenicity.

    β2 effects: increased peripheral vascular resistance, bronchospasm, decreased insulin release, increased bladder and uterine tone

This generic information should be enough to claim a  workmanlike familiarity with the pharmacology of beta-blockers in general. Unfortunately, it appears that the college wanted an in-depth understanding of specific selected drugs. In their comments on Question 10 from the first paper of 2013, CICM examiners hectored the trainees for listing "class specific information  about beta blockers rather than demonstrating an understanding of carvedilol`s particular properties." Thus, the tabulated particular properties of the three drugs from past exam questions are also offered here.

Name Metoprolol Carvedilol Esmolol
Class Beta blocker Beta blocker Beta blocker
Chemistry aryloxypropanolamine aryloxypropanolamine aryloxypropanolamine
Routes of administration Oral or IV Oral IV
Absorption 50% oral bioavailability 25-35% oral bioavailability 0% oral bioavailability
Solubility pKa 9.7, poor lipid solubility pKa 8.77, good lipid solubility pKa 9.5, minimal lipid solubility
Distribution VOD 2.8-4.8 L/kg; only 12% protein bound VOD 2 L/kg; 95% protein bound VOD 3.4 L/kg; 60% protein bound
Target receptor Selective β1 receptor blocker Nonselective β1 and β2 receptor blocker, with some anti-α1 effects; also some sodium channel blocker (membrane stabilising) effects Highly selective β1 receptor blocker
Metabolism Mainly hepatic clearance Mainly hepatic clearance Rapidly metabolised in blood by hydrolysis of methyl ester linkage
Elimination minimal renal excretion; half-life 3-4 hrs minimal renal excretion; half-life 7-10 hrs minimal renal excretion; half-life 9 min
Time course of action 12-24 hrs 12-24 hrs Rapid onset and offset of effect
Mechanism of action By binding to Gs-protein coupled β1 receptors, blocks cAMP synthesis By binding to Gs-protein coupled β1 and β2 receptors, blocks cAMP synthesis By binding to Gs-protein coupled β1 receptors, blocks cAMP synthesis
Clinical effects β1 effects: decreased heart rate, decreased contractility, decreased blood pressure, lower myocardial oxygen demand and increased diastolic coronary filling, and decreased arrhythmogenicity. β1 effects: decreased heart rate, decreased contractility, decreased blood pressure, lower myocardial oxygen demand and increased diastolic coronary filling, and decreased arrhythmogenicity.

β2 effects: increased peripheral vascular resistance, bronchospasm, decreased insulin release, increased bladder and uterine tone

α1 effects: afterload reduction, vasodilation

Membrane-stabilising (sodium channel blocker) effect
β1 effects: decreased heart rate, decreased contractility, decreased blood pressure, lower myocardial oxygen demand and increased diastolic coronary filling, and decreased arrhythmogenicity.
Single best reference for further information Oliver et al (2019) Oliver et al (2019) Oliver et al (2019)
 

Oliver et al (2019) offers an excellent (free) class overview and discusses mechanisms of action. Pharmacokinetics are well covered by Johnsson & Regårdh (1976), McDevitt (1987) and Kendall (1997). In short, these drugs are well-reported and there is no shortage of peer-reviewed material; rather the bigger difficulty was sorting the abundance of data into an easily digestible summary.  Cruickshank (2007) is also excellent for a quick summary of the accepted beneficial effects.

Available beta-blockers

This class of drugs suffers from an overabundance of members. Every man and his dog seem to have brought a beta-blocker to the drug approval authorities at one stage or another. Of these, the following table is a non-exhaustive list, trimmed only by the author's self-interest. 

Beta-blockers,
Arrayed by their year of Market Availability
Drug Year of commercial availability
Pronethalol 1962 (the first beta-blocker)
Propanolol 1965
Atenolol 1976
Labetalol 1977
Metoprolol 1982
Bisoprolol 1986
Esmolol 1987
Carvedilol 1995
Nebivolol 1997

In case one ever needs to classify these, there is in fact a formal classification system available, which is purely functional in nature. This comes from a number of sources, of which the most authoritative is probably the ESC consensus statement (López-Sendó et al, 2004). It groups the drugs by their selectivity for various receptors:

  • Non-selective
    • Propanolol
    • Sotalol
  • β1-selective
    • Atenolol
    • Metoprolol
    • Bisoprolol
    • Nebivolol
    • Esmolol
  • Combined α- and β-blocker effect
    • Carvedilol
    • Labetalol

This is reasonably simple to remember, and accurate enough for government work. In case detail and precision are required, Oliver et al (2019) have a nice table (their Table 1) where even receptor activity (pKD) is listed. There are other classification systems as well, all of which seem to have been required for Question 14 from the second paper of 2019. One could also rank these drugs according to how well they block sodium channels, or according to the presence of intrinsic sympathomimetic activity (ISA), which some of them have. Thus:

Three Different Classification of Beta-blockers
According to selectivity According to membrane stabilising effects According to intrinsic sympathomimetic activity

Non-selective

  • Propanolol
  • Sotalol

β1-selective

  • Atenolol
  • Metoprolol
  • Bisoprolol
  • Nebivolol
  • Esmolol

Combined α- and β-blocker effect

  • Carvedilol
  • Labetalol

Stabilising

  • Propanolol
  • Sotalol
  • Metoprolol

Non-stabilising

  • Atenolol
  • Bisoprolol
  • Nebivolol
  • Esmolol
  • Carvedilol
  • Labetalol

ISA

  • Labetalol
  • Acebutolol
  • Pindolol

Non-ISA

  • Propanolol
  • Atenolol
  • Metoprolol
  • Bisoprolol
  • Nebivolol
  • Esmolol
  • Sotalol
  • Carvedilol

Chemical structure and structure-function relationships

All beta-blockers are basically just derivative variations on one mother sauce recipe, which is the aryloxypropanolamine molecule. From this elemental material, all others are elaborated by the adding of various side chains and extra aromatic rings. Additionally, the suffix "-olol" takes its origin from these substances, supposedly taken from the propan-ol and ethan-ol elements. Following this dangerously overstretched metaphor, the Escoffier of beta blocker chemistry appears to be Sir James Black, who went on to receive the Nobel Prize in Medicine for his work on the development of propanolol.

The best resource for these structure and function relationships actually ended up being an ageing textbook on pharmacology by Gringauz (1997), where beta-blockers are discussed in much better detail (and with more useful detail) than any modern text. Much can be said here, but the most clinically important feature of these drugs (their selectivity) boils down to this:

Structure and function relationships which govern the beta-1selectivity of beta blockers

In short, it appears that most of the selectivity is determined whether the side-chain of the aryl ring is in the -para, -meta or -ortho position. The molecule used here is esmolol, and it has a sidechain in para-position, which makes it highly cardioselective. There are of course many other structural elements which play a major role in the way these drugs are used and administered (eg. hydrophilic side chains which determine lipophilicity, or the features which determine hepatic metabolism), but those are probably not related to their function, i.e. how they interact with their drug target. 

Routes of administration and GI absorption

The general class rule for basically all beta-blockers is relatively poor oral bioavailability. Most of them absorb well but are defenceless again liver enzymes. The upshot of this is the much lower dose used for IV administration for drugs such as metoprolol, which are available as both oral and IV formulations.

Bioavailability of Beta Blockers
Drug Available routes GI absorption Bioavailability pKa
Propanolol Oral > 95% 30% 9.5
Atenolol Oral ~ 50% 45-55% 9.6
Labetalol Oral or IV > 90% 25% 9.3
Metoprolol Oral or IV > 95% 50% 9.7
Bisoprolol Oral > 90% 90% 9.5
Esmolol IV 0%  0% 9.5
Carvedilol Oral > 90% 25-35% 8.8
Nebivolol Oral > 90% 12-96% 8.1

So, the main exception to the rule is esmolol, which is only available as an IV drug. Of the rest, the only standout actor is nebivolol, which has excellent bioavailability in poor CYP2D6 metabolizers (96%), but extremely poor bioavailability (12%) in everybody else. Remarkably, somehow we are not experiencing an epidemic of catastrophic nebivolol toxicity among the slow metabolisers, and "no significant difference has been found between the two profiles in terms of safety and efficacy" (Coats & Jain, 2017).

Solubility and protein binding

The lipid solubility of beta-blockers is a determinant of several important features, not the least of which being their side-effects and hepatic metabolism. Lipid solubility is usually expressed as the partition between an organic solvent and an aqueous solvent, as was done here (parroting McDevitt, 1987). Unfortunately,  objective data on this was not available for each agent, and under some circumstances, one has to make do with vague statements (like "this drug is highly lipid-soluble") from PI documents. 

Solubility and Protein Binding of Beta Blockers
Drug Lipid solubility  Protein binding
Propanolol 20.2 (highest lipid solubility among β-blockers) 93%
Atenolol 0.015 3%
Labetalol  lipid soluble 50%
Metoprolol 0.98 12%
Bisoprolol 4.8 30%
Esmolol very poor lipid solubility 60%
Carvedilol highly lipid soluble 95%
Nebivolol highly lipid soluble 98%

Lipophilicity has various important implications for the properties of these agents (Cruickshanks, 1980).  In short, higher lipophilicity leads to:

  • better GI absorption (eg. the absorption of atenolol is usually incomplete, only 50%)
  • a shorter plasma half-life (mainly due to rapid distribution)
  • Greater protein binding (which means less predictable pharmacokinetics during critical illness)
  • higher tissue penetration (eg. the most lipid-soluble beta-blocker is propanolol, which means it penetrates the blood-brain barrier most readily (and it is debated whether this characteristic also promotes more side effects)

Distribution, metabolism and elimination

Basically, they are all reasonably widely distributed and dependent to a considerable extent upon hepatic metabolism,  with the exception of atenolol which is confined basically to body water, and which is eliminated by the kidneys in a totally unchanged form.

Distribution, Metabolism and Elimination of Beta Blockers
Drug VOD Hepatic metabolism Renal excretion (%)
Propanolol 4 L/kg Mainly hepatic clearance minimal
Atenolol 0.3-0.6 L/kg 0% 100%
Labetalol 3-16 L/kg Mainly hepatic clearance minimal
Metoprolol 2.8-4.8 L/kg Mainly hepatic clearance minimal
Bisoprolol 3.5 L/kg 50% 50%
Esmolol 3.4 L/kg Mainly hepatic clearance minimal
Carvedilol 2 L/kg Mainly hepatic clearance minimal
Nebivolol 11.2 L/kg Mainly hepatic clearance minimal

This has clinical implications, elaborated further by Borchard (1990):

  • You'd have to adjust the dose of atenolol (and sotalol) to compensate for impaired renal function. Which realistically means that you would probably just choose essentially any other beta-blocker, because...
  • The rest of the beta-blockers are mainly metabolised by the liver, into inactive metabolites.

Half life and duration of effect

Unless otherwise specified, these data come from Feeley et al (1983). The most important take-home message here is the complete lack of correlation between the plasma elimination half-life and the duration of effect, which for most of these drugs is much longer. For example, Johannson et al (1980) found that the clinical effects of metoprolol and propanolol were still essentially the same at 24 hours after the last dose. 

Half-Life of Beta Blockers
Drug Half life
Propanolol 2-6 hrs
Atenolol 6-9  hrs
Labetalol 3-4  hrs
Metoprolol 3-4  hrs
Bisoprolol 9-12 hrs
Esmolol 9 min
Carvedilol 7-10 hrs
Nebivolol 10-30 hrs

Again, esmolol is a dramatic exception, in that it's half life in the blood is approximately 9 minutes.

Molecular drug target

All of these drugs bind to the β1 and βreceptors with varying degrees of affinity. The effects of activating catecholamine receptors is detailed elsewhere, and so here it will suffice to summarise by saying that these are all  Gs-protein coupled receptors. They normally bind catecholamines such as adrenaline and noradrenaline, which increases the synthesis of cAMP and produce downstream effects (like speeding up intrinsic pacemaker tissue or increasing the contractility of cardiac myocytes). Ergo, beta-blockade decreases cAMP concentration and counteracts all of these downstream effects.

Each mentioned beta-blocker has a different affinity for the different classes of receptor, which is usually expressed as a dissociation constant (Kd).  As you might recall, Kd is the rate constant of dissociation at equilibrium, defined as the ratio koff / kon; and so when Kd is high, it means that a large concentration of the drug is required to occupy 50% of the receptors, i.e. the drug and the receptor have a low affinity for one another. Confusingly, here are some -log(pKd) values from Oliver et al (2019), where the higher number means a higher affinity.

β-receptor Affinities of Beta Blockers
Drug β1 receptor affinity β2 receptor affinity Selectivity ratio
Propanolol 8.16 8.44 0.52
Atenolol 6.41 5.09 20.89
Labetalol 7.63 8.03 0.4
Metoprolol 7.26 5.49 58.88
Bisoprolol 7.43 5.42 102.33
Esmolol 6.94 5.20 52.48
Carvedilol 8.75 8.96 0.62
Nebivolol 8.79 6.65 138.04

Now, these are base 10 log(Kd) values, and so the better value to look at would be the selectivity ratio, as that probably makes more intuitive sense. The reader is reminded that the original article did not present these values, i.e. the author calculated them himself, with his own puny maths powers, which may have resulted in a factor-of-ten error (or worse). In short, don't quote that column in your exam answers. If correct, it represents the ratio of βto β2 affinity. In other words, where the selectivity ratio of propanolol is 0.5, it means it has about 50% less affinity for the βreceptor than for β2.  The same sort of low sub-1.0 values are seen for the other "non-selective" beta-blockers, such as labetalol and carvedilol. In contrast. bisoprolol has a ratio of 102.33 which means it is about 100 times more selective for the β1 receptor. As you can see, nebivolol and bisoprolol have some of the highest cardioselectivity among these drugs. Additionally, you can use the -log(pKd) values to compare between drugs; for instance, metoprolol with its -log(pKd) value of 7.26 has about 34 times less affinity for the βreceptor than does carvedilol (8.75). 

Interestingly (and unsurprisingly) minor variations in how you test these receptor-ligand interactions will give you wildly different values, eg. Oliver et al (2019),  Baker (2005) and Baker (2017) all give completely different selectivity ratios. It does not help that some studies are in humans, and some in rats. The esmolol data in the table above appears to have originated from guinea pig studies

To make things more complicated,  some beta-blockers have intrinsic sympathomimetic activity, i.e. they act as agonists or partial agonists. There are many such drugs, but none of them made the exclusive list in this chapter, as they are either ancient, not available in Australia, or presented exclusively in the form of eyedrops. In case a list is required for some reason, here is one extracted from a table in Feeley et al (1983):

  • Oxprenolol
  • Pindolol
  • Timolol
  • Acebutolol
  • Practolol

Nebivolol is apparently also a partial β2-agonist (Erickson et al, 2013), which adds to its positive vasodilatory afterload-reducing effects, and probably makes it more attractive to patients who are prone to bronchospasm (or who have severe peripheral vascular disease).

Additionally, some of these agents have some anti-α effects, which give them afterload reducing properties. There are really two agents which fall into this category, those being labetalol and carvedilol. Coming from the perspective of "what do I really need to know about this", Wong et al (2015) report that these drugs are still much better as beta-blockers than they are as alpha-blockers, i.e. by the time you start seeing a real measurable drop in blood pressure due to vasodilation, your the βreceptor effects are already quite prominent, i.e the patient is so bradycardic that you can't bring yourself to increase the dose any further. 

Lastly, some beta-blockers have an intrinsic "membrane stabilising" effect which is completely unrelated to their beta-blocking properties, and which appears to be a Class I antiarrhythmic effect. A surprisingly large number of them have this property, according to Aronson (2008). Perhaps by coincidence, they are also the non-selective agents: 

  • Propanolol
  • Carvedilol
  • Labetalol

The most important thing to remember here is that none of these agents have a clinically relevant sodium channel blocker effect within their normal dose range. In other words, you would only ever see these effects in the context of a severe overdose.

Mechanism of action

Effect on heart rate: The lowering of heart rate is probably the most therapeutically important effect of beta-blockade, so it makes sense to know a little bit about the mechanism of this. In short, it is the specific effect of blocking the  β1 receptors on the duration of the action potential of pacemaker cells.  Noma (1996) explains these mechanisms very well, and they are covered to some minor extent in the chapter dealing with spontaneous cardiac electrical activity. Catecholamines influence pacemaker cell depolarisation by altering the "funny current", If This is the constant inward sodium / outward potassium flow which occurs via HCN channels (hyperpolarization-activated, cyclic-nucleotide–gated), which are controlled by intracellular cAMP concentrations (hence the "cyclic-nucleotide–gated" bit).

So: decrease the concentration of cAMP by blocking the  β1 receptor, and the If current slows down, making the pacemaker cells depolarise less frequently. One might think that somebody at some stage might have recorded such a slowed action potential, but no - wherever you see this mentioned, they generally reproduce this image from Difrancesco (1993), which demonstrates a rate increase with a nonselective β1 receptor agonist (isoprenaline).

changes in pacemaker current with isoprenaline and acetylcholine

Effect on contractility: The inotropic effects of systemic catecholamines and of the sympathetic nervous system is mediated by the β-1 receptors, which are Gs-protein coupled receptors. The increase in cyclic AMP which results from their activation increases the activity of protein kinase A, which in turn phosphorylates calcium channels. Calcium influx ensues, and with more calcium available intracellularly, the cardiac contractility increases. Thus, by dampening this cAMP-mediated calcium orgy,  conventional wisdom suggests that beta-blockers should probably reduce cardiac contractility.

They probably do, but not by very much, and the effect tends to be obscured by their effect on the heart rate, which typically drops - resulting in increased LV diastolic filling time, a higher end-diastolic volume, and therefore higher contractility. To eliminate this influence, Bourdillon et al (1979) gave healthy volunteers about 0.1mg/kg of IV metoprolol while they were being atrially paced. They observed a 10% fall in mean left ventricular dP/dT, which is a reasonable measure of contractility.

Effect on blood pressure:  Yes, these drugs are grouped with antihypertensives, and yes they do produce a reasonably predictable decrease in BP. However, it is not massive. For example, Sannerstedt & Wasir (1977) gave 0.15mg/kg of metoprolol to hypertensive volunteers, and found their resting systolic dropped from about 150m mmHg to about 140 mmHg. In the abovementioned paper by Bourdillon et al (1979), there was little change in blood pressure while the patients were atrially paced.

Effect on myocardial oxygen consumption: So, now that you are beta-blocked,  your heart rate is lower, your afterload is (slightly) reduced, and your contractility is decreased. All the main determinants of myocardial oxygen demand are therefore controlled. In addition, you now get a longer diastolic coronary filling time. In short, you should expect beta-blockers to have a highly positive effect in any scenario where myocardial oxygen supply is stressed. That was indeed illustrated by such studies as MIAMI (metoprolol) and ISIS-1 (atenolol), which demonstrated a 13-15% reduction in in-hospital cardiovascular mortality for patients treated with a beta-blocker after their acute MI.

Effect on arrythmogenicity: beta-blockers act as antiarrhythmics, which is a topic for a whole separate discussion.  Reiter & Reiffel (1998) listed the following factors as contributing to these effects:

  • Decrease in the automaticity of ectopic pacemakers (thus, less arrhythmogenesis)
  • Prolonged refractory period for all excitable myocardial tissues. (thus, reduced propagation of malignant arrhythmias)
  • Decrease in ventricular fibrillation threshold
  • Prevention of a catecholamine reversal of concomitant class I/III antiarrhythmic drug effects
  • Reversal of ischaemia/reperfusion induced proarrhythmic tendency by their effects on myocardial oxygen supply and demand

Clinical effects

In summary, beta blocker effects are:

  • β1 receptor blockade
    • slows sinoatrial node
    • decelerates ectopic pacemakers
    • decreases contractility of the heart
    • decreases lusitropy
    • decreases renin release by the kidney
  • β2 receptor blockade
    • Opposes the relaxation of vascular smooth muscle in skeletal muscle arterioles, and therefore increases systemic vascular resistance
    • Opposes the relaxation of bronchiolar smooth muscle
    • Contracts gut wall smooth muscle
    • Contracts the bladder wall
    • Contracts the pregnant uterus
    • Decreases gluconeogenesis and glycogenolysis in the liver
    • Decreases insulin release

References

Cruickshank, J. M. "Are we misunderstanding beta-blockers." International journal of cardiology 120.1 (2007): 10-27.

López-Sendó, José, et al. "Expert consensus document on β-adrenergic receptor blockers: The Task Force on Beta-Blockers of the European Society of Cardiology." European heart journal 25.15 (2004): 1341-1362.

Oliver, Eduardo, Federico Mayor Jr, and Pilar D’Ocon. "Beta-blockers: Historical perspective and mechanisms of action." Revista Española de Cardiología (English Edition) 72.10 (2019): 853-862.

Gorre, Frauke, and Hans Vandekerckhove. "Beta-blockers: focus on mechanism of action Which beta-blocker, when and why?." Acta cardiologica 65.5 (2010): 565-570.

Johnsson, Gillis, and C-G. Regårdh. "Clinical pharmacokinetics of β-adrenoceptor blocking drugs." Clinical pharmacokinetics 1.4 (1976): 233-263.

Kendall, Martin J. "Clinical relevance of pharmacokinetic differences between beta blockers." The American journal of cardiology 80.9 (1997): 15J-19J.

McDevitt, D. G. "Comparison of pharmacokinetic properties of beta-adrenoceptor blocking drugs." European heart journal 8.suppl_M (1987): 9-14.

Coats, A., and S. Jain. "Protective effects of nebivolol from oxidative stress to prevent hypertension-related target organ damage." Journal of human hypertension 31.6 (2017): 376-381.

Gengo, Francis M., Larry Huntoon, and William B. McHugh. "Lipid-Soluble and Water-Soluble ß-Blockers: Comparison of the Central Nervous System Depressant Effect." Archives of internal medicine 147.1 (1987): 39-43.

Borchard, U. "Pharmacokinetics of beta-adrenoceptor blocking agents: clinical significance of hepatic and/or renal clearance." Clinical physiology and biochemistry 8 (1990): 28.

Cruickshank, John M. "The clinical importance of cardioselectivity and lipophilicity in beta blockers." American Heart Journal 100.2 (1980): 160-178.

Feely, John, Philip J. de Vane, and Derek Maclean. "New Drugs. Beta-blockers and sympathomimetics." British medical journal (Clinical research ed.) 286.6370 (1983): 1043.

Baker, Jillian G. "The selectivity of β‐adrenoceptor antagonists at the human β1, β2 and β3 adrenoceptors." British journal of pharmacology 144.3 (2005): 317-322.

Baker, Jillian G., et al. "Novel selective β1‐adrenoceptor antagonists for concomitant cardiovascular and respiratory disease." The FASEB Journal 31.7 (2017): 3150-3166.

Wong, Gavin WK, Alexandra Laugerotte, and James M. Wright. "Blood pressure lowering efficacy of dual alpha and beta blockers for primary hypertension." Cochrane Database of Systematic Reviews 8 (2015).

Aronson, Jeffrey K. "Changing beta-blockers in heart failure: when is a class not a class?." (2008): 387-389.

Noma, Akinori. "Ionic mechanisms of the cardiac pacemaker potential." Japanese heart journal 37.5 (1996): 673-682.

Dilaveris, Polychronis, et al. "Heart rate lowering by inhibition of the pacemaker current: a new therapeutic perspective in cardiovascular disease." Cardiovascular & Hematological Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Cardiovascular & Hematological Agents) 4.4 (2006): 313-318.

Erickson, Catherine E., et al. "The β-blocker nebivolol is a GRK/β-arrestin biased agonist." PloS one 8.8 (2013): e71980.

DAVIS, LARRY D., and JOHN V. TEMTE. "Effects of propranolol on the transmembrane potentials of ventricular muscle and Purkinje fibers of the dog." Circulation Research 22.5 (1968): 661-677.

Sannerstedt, R., and H. Wasir. "Acute haemodynamic effects of metoprolol in hypertensive patients." British Journal of Clinical Pharmacology 4.1 (1977): 23-26.

Bourdillon, Patrick D., Rudolph Canepa-Anson, and Anthony F. Rickards. "Hemodynamic effects of intravenous metoprolol." The American journal of cardiology 44.6 (1979): 1195-1200.

MIAMI Trial Research Group. "Metoprolol in acute myocardial infarction (MIAMI). A randomised placebo-controlled international trial." European Heart Journal 6.3 (1985): 199-226.

ISIS-1 (First International Study of Infarct Survival) Collaborative Group. "Randomised trial of intravenous atenolol among 16,027 cases of suspected acute myocardial infarction: ISIS‐1." Lancet 2.8498 (1986): 57-66.

Pratt, Craig, and EDGAR LICHSTEIN. "Ventricular antiarrhythmic effects of beta‐adrenergic blocking drugs: A review of mechanism and clinical studies." The Journal of Clinical Pharmacology 22.8‐9 (1982): 335-347.

Haverkamp, Wilhelm, Gerhard Hindricks, and Hartmut Gülker. "Antiarrhythmic properties of beta-blockers." Journal of cardiovascular pharmacology 16 (1990): S29-32.

Reiter, Michael J., and James A. Reiffel. "Importance of beta blockade in the therapy of serious ventricular arrhythmias." The American journal of cardiology 82.4 (1998): 9I-19I.

Kostis, John B. "Beta-blocker duration of action and implications for therapy.The American journal of cardiology 66.16 (1990): G60-G62.

Johansson, S. R., et al. "Duration of action of beta blockers." Clinical Pharmacology & Therapeutics 27.5 (1980): 593-601.