This chapter is related to the aims of Section F11(iii) from the 2017 CICM Primary Syllabus, which expects the exam-going trainees to be able to "outline the pharmacology of drugs used to treat acute pulmonary hypertension".  This chapter on the pharmacology of pulmonary vasodilators is separated from nitric oxide for no specific reason, other than the author's desire to cover that gas in some detail, compared to the lack of such desire for other pulmonary vasodilators. To be fair, Question 14 from first paper of 2011 was the only Part One question which asked about this topic, and it was quite NO-centric. The other pulmonary vasodilator which can be given in the inhaled manner are nebulised prostacycline and, to a lesser extent, oxygen. Beyond the CICM question, the syllabus does not specify that only "inhaled" vasodilators will be asked about, and so this chapter also needs to cover some of the non-inhaled substances. Though of course that distinction is quite artificial, because just about anything can be inhaled even though it might not be ordinarily meant for inhalational use.  
These substances will be covered here in some bare minimum of detail. Contrary to popular belief among the senior members of college faculties, rote learning facts about drugs is not conducive to the understanding of their function or to understanding the principles of their safe responsible use. However, some remembered facts were apparently expected by the examines in Question 14. In short, "accurate detail concerning the receptor and second messenger effects of drugs was expected. The importance of V/Q matching and reduction in systemic effects via inhalational administration needed to be stated. Better answers included discussion of serious adverse effects". For the best single reference, it is difficult to go past "Pulmonary vasodilators" by Mark S. Siobal (2007), but realistically even this single article may be excessive.
In briefest summary:
Inhaled Pulmonary Vasodilators
Name Nitric oxide Epoprostenol
Class Inhaled pulmonary vasodilator Inhaled pulmonary vasodilator
Chemistry A free radical with the formula NO Synthetic analogue of the naturally occurring eicosanoid prostacyclin (prostaglandin I2 or PGI2)
Routes of administration Administered as part of inspired gas mixture, usually as an admixture fraction measured in tens of ppm, via a proprietary system (INOMax) Can be intravenous, but usually nebulised as a part of a solution with a glycine buffer, using a continuous ultrasonic nebuliser
Absorption Absorbs rapidly into the pulmonary circulation via the lungs Absorbs rapidly into the pulmonary circulation via the lungs
Solubility As it dissociates in water, nitric oxide produces nitric acid (HNO3) which has a pKa of -1.3 Natural pKa is 4.4; requires a diluent which contains glycine and sodium hydroxide. The pH of the reconstituted drug mixture has a pH of around 12, because the drug tends to spontaneously hydrolyse in aqueous solution at a normal pH
Distribution VOD is impossible to measure, but is potentially very large. NO reacts with oxygen and water to produce nitrogen dioxide and nitrites, which then bind to haemoglobin and produce either nitrosylhaemoglobin or methaemoglobin, i.e. it can be described as "highly protein bound". 0.357L/kg
Target receptor Soluble guanylyl cyclase (which is induced by NO) Activates G protein-coupled PGE receptors on platelets and endothelial cells, which activates adenlyl cyclase and increases cAMP
Mechanism of action Inhibits vasoconstriction by increasing the amount of cyclic GMP (cGMP) in the cytosol, thus decreasing the amount of cytosolic calcium ions available to sustain smooth muscle contraction Increased cyclic AMP leads to decreased platelet activation and activates PKA, which phosphorylates and inhibits myosin light-chain kinase which leads to smooth muscle relaxation and vasodilation
Metabolism One way or another, nitric oxide ends up as methaemoglobin and nitrate. Either it reacts with lung water, becoming nitrite (which reacts with oxyhemoglobin and generates methaemoglobin and nitrate) or it combines directly with oxyhaemoglobin, with the same results. If it encounters hypoxic blood, it can combine with deoxyhaemoglobin to create nitrosyl-haemoglobin, which then rapidly becomes methaemoglobin when it contacts oxygen. Degrades spontaneously as well as enzymatically into about sixteen major and minor metabolites
Elimination Nitrates are eliminated mainly in urine whereas methaemoglobin is metabolised in several hours into
haemoglobin by endogenic reductases. The nitrates excreted in urine represent over 70% of the inhaled NO
Half-life is about six minutes
Time course of action Onset of effect is seen within seconds Platelet inhibition effects last up to 2 hrs; smooth muscle vasodilation is very shortlived (comparable with half-life)
Clinical effects Apart from pulmonary vasodilation, there is methemoglobinaemia, hypotension (maybe some of it does leak into the systemic circulation, or maybe this the effect of depressed LV function, rebound hypoxia after abrupt withdrawal, thrombocytopenia (in as many as 10% of patients) and increased susceptibility to pulmonary infections probably due to NO2 formation and associated lung injury. Vasodilation (pulmonary as well as systemic); inhibition of platelet aggregation; facial flushing, tachycardia, bronchodilation, inhibition of gastric acid secretion, and decreased gastric
Single best reference for further information TGA (AusPAR) product information Flolan PI by GlaxoSmithCline
In addition to this, the college examiners complained in their comments to Question 14 from first paper of 2011 that  "many candidates neglected to include oxygen which is also a drug with significant pulmonary vasodilating properties." Fine, here's a table of its pharmacological properties. However, it must be pointed out that it prevents or reverses vasoconstriction, i.e it does not have a very potent vasodilator function for patients whose oxygenation is normal. Of course that's quite a pointless comment to make, considering one would never be giving oxygen to those people. In the hypoxic population, restoring normal oxygenation can halve pulmonary vascular resistance, according to Roberts et al (2001)
Name Oxygen
Class Vital ingredient of multicellular life
Chemistry Diatomic gas
Routes of administration Inhaled; also as intravenous or intra-arterial infusion of well-oxygenated blood (i.e. ECMO), and can be given externally (as in hyperbaric oxygen therapy)
Absorption Absorbs rapidly into the pulmonary circulation via the lungs (250ml/min with 21% FiO2, at rest)
Solubility Poor solubility in water
Distribution Widely distributed- total body oxygen content is 64% by weight. Highly protein-bound (to haemoglobin)
Target receptor Cytochrome c mitochondrial enzymes
Mechanism of action Increases ATP by acting a substrate for aerobic metabolism of glucose
Metabolism Metabolised in all tissues (mainly brain, heart and skeletal muscle)
Mainly metabolised by cytochrome c mitochondrial enzymes (90%)
Zero-order clearance kinetics, roughly 200ml/minute
Elimination Rapidly eliminated by metabolism into CO2 and water
Time course of action Minutes
Clinical effects Drying of mucous membranes and inspissation of secretions
Inflammatory tracheobronchitis
Decreased central respiratory drive (minimally)
Hypecapnoea in "CO2 retainers" mainly by virtue of V/Q mismatch and Haldane effect
Absorption atelectasis
Increased left-to-right shunting in ASDs
Increased peripheral vascular resistance
Cerebral and coronary vasoconstriction
Retrolental fibroplasia of the newborn
Decreased erythropoiesis
Single best reference for further information Haim Bitterman's 2009 article, "Bench-to-bedside review: oxygen as a drug"

Other pulmonary vasodilators

Non-inhaled pulmonary vasodilators also exist, and they can be broadly separated into two groups: phosphodiesterase inhibitors and endothelin receptor antagonists.
A Comparison of Phosphodiesterase Inhibitors
Name Sildenafil Tadalafil Milrinone
Class Pulmonary vasodilator Pulmonary vasodilator Ino-dilator
Chemistry Pyrazolopyrimidine Pyrazinopyridoindole Biperidine
Routes of administration Oral, but also available as an attractive buccal spray Oral IV; but can also be administed as a nebulised aerosol, and had initially been marked as an oral preparation
Absorption Rapidly absorbed after oral administration, with a mean absolute bioavailability of 41%; a high-fat meal reduces absorption Appears to have a high oral bioavailability Well absorbed orally; 92% oral bioavailability
Solubility Basic drug, with a pKa value of 8.7; highly (96%) protein-bound Acidic drug, pKa ~ 3.5; highly protein bound (94%) pKa 4.6 and 8.5; good solublity at physiological pH
Distribution Very large VOD: 105 L/kg; i.e extensively distrbuted into tissues very large VOD: 64 L/kg 0.38L/kg; 70% protein bound
Target receptor Phosphodiesterase 5 Phosphodiesterase 5 Phosphodiesterase 3
Mechanism of action Increases cyclic GMP by inhibiting phosphodiesterase 5, which is responsible for cGMP catabolism. Selective for vascular smooth muscle. Increases cyclic GMP by inhibiting phosphodiesterase 5, which is responsible for cGMP catabolism. Selective for vascular smooth muscle. Increases cyclic AMP by inhibiting phosphodiesterase 3, which is responsible for cAMP catabolism. Selective for vascular smooth muscle and cardiac muscle.
Metabolism Cleared predominantly by the liver: metablised by CYP3A into an active metabolite which itself has about 50% of th PDE5-inhibiting potency of the parent drug Hepatic clearance is the main mode; metabolised by CYP 3A4 into a totally inactive metabolite Mostly cleared renally; of the free fraction some undergoes hepatic metabolism into an inactive o-glucouronide, and the rest is excreted unchanged at a rate which varies depending on renal blood flow
Elimination Half life of sildenafil and its major metabolite is about 4 hours. Half-life is 17.5 hrs Half life is 2.3 hours in patients with heart failure, slightly less in normal healthy adults and longer in patients with renal dysfunction
Time course of action Vasodilation is maximal approximately 1-2 hours after dosing Effects are maximal ~ 2 hours following oral administration Onset of action is usually within 5-15 minutes
Clinical effects Pulmonary vasodilation, systemic vasodilation with hypotension, reflex tachycardia in reponse to this; priapism; headache Pulmonary vasodilation, systemic vasodilation with hypotension, reflex tachycardia in reponse to this; priapism; headache Improved vntricular contractility; decreased systemic vascular resistance; decreased pulmonary vascular resistance; tachycardia; propensity to arrhythmias.
Single best reference for further information REVATIO product pamphlet (sildenafil citrate) TGA product information for Cialis, by Eli Lily Canadian (Novopharm) product pamphlet for milrinone lactate

For completeness, the following table contains details about the two most popular endothelin-1 receptor antagonists. There is no possible way this could ever be viewed as core material even by the most deranged examiners. The only halfway interesting thing to remember about these drugs is the fact that, unlike the vast majority of drugs, they are excreted almost exclusively via the bile.

Name Bosentan Ambrisentan
Class Pulmonary vasodilator Pulmonary vasodilator
Chemistry Pyrimidine derivative Propionic acid
Routes of administration Oral Oral
Absorption 50% oral bioavailability 90% oral bioavailability
Solubility pKa 5.8; highly protein-bound (98%); 99% protein bound; this drug is a carboxylic acid with a pKa of 4
Distribution Large VOD, 28L/kg Large VOD, 40L/kg
Target receptor Endothelin receptor types ETA and ETB Endothelin receptor types ETA and ETB
Mechanism of action

Bosentan is a non-selective ETA and ETB receptor antagonist.

By competitive inhbition, bosentan  prevents binding of endothelin to its ETA receptor, which would usually produce smooth muscle contraction (vasoconstriction). ETA are G-protein coupled receptors; activating them leads to an increase in cAMP and an increased availability of intracellular calcium which gives rise to vasoconstriction. 

Ambrisentan is a selective ETA receptor antagonist.

By competitive inhbition, ambrisentan prevents binding of endothelin to its ETA receptor, which would usually produce smooth muscle contraction (vasoconstriction). ETA are G-protein coupled receptors; activating them leads to an increase in cAMP and an increased availability of intracellular calcium which gives rise to vasoconstriction.

Metabolism Hepatic metabolism and almost completely biliary elimination; only 3% of the dose is recovered from the urine. Only of the metabolites is effective and probably contributes 10-20% of the total drug effect Ambrisentan is excreted largely unchanged (45.6% of the dose); excretion is biliary. The rest is glucouronidated and oxidised by CYP3A4 into relatively inactive metabolites
Elimination Terminal elimination half-life is about 5 hours in healthy adult subjects In humans, the terminal half-life following oral administration was determined to be approximately 15 hours
Time course of action Effects are maximal ~ 1 hour following oral administration Maximum plasma concentrations (Cmax) of ambrisentan typically occur around 1.5 hours post dose
Clinical effects Pulmonary vasodilation, fluid retention, pulmonary veno-occlusive disease, foetal toxicity, anaemia Pulmonary vasodilation, fluid retention, pulmonary veno-occlusive disease, foetal toxicity, anaemia, LFT derangedment ("transaminitis"),
Single best reference for further information FDA information on the TRACLEER brand of bosentan TGA information pamphlet for the VOLIBRIS brand of ambrisentan


Roberts, David H., et al. "Oxygen therapy improves cardiac index and pulmonary vascular resistance in patients with pulmonary hypertension." Chest 120.5 (2001): 1547-1555.

Siobal, Mark S. "Pulmonary vasodilators." Respiratory care 52.7 (2007): 885-899.